大鼠白介素12Elisayabo官网手机版 盒,BR

中文名称： 大鼠白细胞介素12检测yabo官网手机版 盒
英文名称： Rat IL-12 ELISA Kit
别名： IL12A; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit;CLMF p35
储存条件： 2-8℃，6 months
规格： 48T ; 96T
检测目标： IL-12
样品类型： 血清/血浆/细胞培养上清
反应性： Rat
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 62.5-4000 pg/ml
灵敏度： 31.25 pg/ml
特异性： 不与大鼠IL-12类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3.5h

检测原理： Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠IL-12单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠IL-12会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠IL-12抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠IL-12发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠IL-12，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠IL-12浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠IL-12的浓度。
背景说明： Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL 12 R beta 1) and a 130 kDa signal transducing subunit (IL 12 R beta 2). IL 12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells.

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