大鼠白介素18Elisayabo官网手机版 盒,BR

中文名称： 大鼠白细胞介素18检测yabo官网手机版 盒
英文名称： Rat IL-18 ELISA Kit
别名： IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; Interleukin-1 gamma
储存条件： 2-8℃，6 months
规格： 48T ; 96T
检测目标： IL-18
样品类型： 血清/血浆/细胞培养上清
反应性： Rat
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 62.5-4000 pg/ml
灵敏度： 31.25 pg/ml
特异性： 不与大鼠IL-18类似物等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Swiss Prot： P97636
Gene ID： 29197
检测原理： Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠 IL-18单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠 IL-18会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠 IL-18抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠 IL-18发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠 IL-18，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠 IL-18浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠 IL-18的浓度。
背景说明： Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response. Rat IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region. Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18. Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60-64% aa sequence identity with human, canine, feline, porcine, and rhesus macaque IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells. Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity. IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta. The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling. IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity. IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma. IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses. Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome. In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion.

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