小鼠肿瘤坏死因子受体超家族，成员1b检测yabo官网手机版 盒,BR

中文名称： 小鼠肿瘤坏死因子受体超家族成员1b检测yabo官网手机版 盒
英文名称： Mouse TNFR2/TNFRSF1B ELISA Kit
别名： CD120b; Etanercept; p75 TNF receptor; p75TBPII; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFR-II; TNFRSF1B
储存条件： 2-8℃，6 months
规格： 96T ; 48T
检测目标： TNFR2/TNFRSF1B
样品类型： 血清/血浆/细胞培养上清
反应性： Mouse
应用： ELISA
反应类型： Sandwich-ELISA
定量/定性： 定量检测
检测范围： 9.375-600 pg/ml
灵敏度： 4.8 pg/ml
特异性： 不与C10,Eotaxin,G-CSF,GM-CSF,IFN-γ,IL-1α,IL-1β,IL-1ra,IL-2,IL-3,IL-4,IL-5,IL-6,IL-7,IL-9,IL-10,IL-10R,IL-12,IL-13,IL-17,IL-18,JE/MCP-1,KC,Leptin,LIF,M-CSF,MIP-1α,MIP-1β,MIP-2,OSM,SCF,TNF-α,TNFRI,Tpo,VEGF等反应。
精密度： 板内，板间变异系数均<10%
回收率： 80%-120%
反应时间： 3h

Swiss Prot： P25119
Gene ID： 21938
检测原理： Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠TNFR2/TNFRSF1B单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠TNFR2/TNFRSF1B会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗小鼠TNFR2/TNFRSF1B抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠TNFR2/TNFRSF1B发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠TNFR2/TNFRSF1B，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠TNFR2/TNFRSF1B浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠TNFR2/TNFRSF1B的浓度。
背景说明： Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

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