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小鼠甲胎蛋白检测yabo官网手机版 盒,BR
Mouse α-fetoprotein/AFP ELISA KIT
(订货以英文名为准)
产品编号:AC16708
品牌:Acmec
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中文名称:
小鼠甲胎蛋白检测yabo官网手机版
盒
英文名称:
Mouse α-fetoprotein/AFP ELISA KIT
保存条件:
2-8℃
中文名称: 小鼠甲胎蛋白检测yabo官网手机版
盒
英文名称: Mouse AFP ELISA Kit
别名: AFP; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
储存条件: 2-8℃,6 months
规格: 48T ; 96T
检测目标: AFP
样品类型: 血清/血浆/细胞培养上清
反应性: Mouse
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 62.5-4000 pg/ml
灵敏度: 31.25 pg/ml
特异性: 不与小鼠Leptin,Prolactin,Resistin,VitaminDBP,人AFP等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3h Swiss Prot: P02772
Gene ID: 11576
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠AFP单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的小鼠AFP会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗小鼠AFP抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠AFP发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的小鼠AFP,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的小鼠AFP浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠AFP的浓度。
背景说明: alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
英文名称: Mouse AFP ELISA Kit
别名: AFP; alpha-1-fetoprotein; Alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
储存条件: 2-8℃,6 months
规格: 48T ; 96T
检测目标: AFP
样品类型: 血清/血浆/细胞培养上清
反应性: Mouse
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 62.5-4000 pg/ml
灵敏度: 31.25 pg/ml
特异性: 不与小鼠Leptin,Prolactin,Resistin,VitaminDBP,人AFP等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3h Swiss Prot: P02772
Gene ID: 11576
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠AFP单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的小鼠AFP会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗小鼠AFP抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的小鼠AFP发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的小鼠AFP,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的小鼠AFP浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠AFP的浓度。
背景说明: alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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