Recombinant Human TNF/TNFa/TNFSF2(C-polyHis),BR

别名： Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;TNF;TNFA;TNFSF2
英文名称： Recombinant Human TNF/TNFa/TNFSF2(C-polyHis)
中文名称： 重组人TNF/TNFa/TNFSF2(C-polyHis)
规格： 10ug ; 50ug ; 500ug ; 1mg
外观(性状)： Lyophilized from a 0.2 μm filtered solution of 20mM PB，150mM NaCl,pH7.4.
纯度： ≥95% as determined by reducing SDS-PAGE.
储存条件： Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
运输条件： 冷藏运输

背景说明： Tumor Necrosis Factor-α (TNF-α) is secreted by macrophages，monocytes，neutrophils，T-cells，and NK-cells following stimulation by bacterial LPS.Cells expressing CD4 secrete TNF-α while cells that express CD8 secrete little or no TNF-α.Synthesis of TNF-α can be induced by many different stimuli including interferons，IL2，and GM-CSF.The clinical use of the potent anti-tumor activity of TNF-α has been limited by the proinflammatory side effects such as fever，dose-limiting hypotension，hepatotoxicity，intravascular thrombosis，and hemorrhage.Designing clinically applicable TNF-α mutants with low systemic toxicity has been of intense pharmacological interest.Human TNF-α that binds to murine TNF-R55 but not murine TNF-R7，exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-α，which binds to both murine TNF receptors.Based on these results，many TNF-α mutants that selectively bind to TNF-R55 have been designed.These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo.Recombinant Human TNF-α High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8，Lys9，Arg10 and Phe157.This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
来源： E.coli

溶剂： Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
内毒素： Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test.

Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus.

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